DNA ligases seal 5′-PO4 and 3′-OH polynucleotide ends by using a few nucleotidyl transfer actions involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are necessary guardians of genomic integrity, and ligase dysfunction underlies human genetic illness syndromes. Crystal buildings of DNA ligases bound to nucleotide and nucleic acid substrates have illuminated how ligase reaction chemistry is catalyzed, how ligases understand harmed DNA ends, And the way protein area actions and Lively-site remodeling are accustomed to choreograph the end-joining pathway.
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